| Extension | Description | Direction | Based on |
| Direct data exchange | |||
| MATLAB | Exchange with MATLAB main workspace | Import/Export | MATLAB core |
| Fiji/ImageJ | Exchange with Fiji | Import/Export | MIJ |
| Imaris | Import from Imaris | Import/Export | IceImarisConnector |
| Omero Server | Import from Omero server | Import | OMERO MATLAB bindings |
| Web URL | Import images using the web URL | Import | MATLAB core |
| System clipboard | Import images from system clipboard | Import | imclipboard |
| Own custom readers/writers | |||
| AM | Amira Mesh, a common file format with Amira | Import/Export | own |
| HDF5 | Hierarchical Data Format 5 | Import/Export | own |
| mibImg | Images in MATLAB format used for Deep Learning workflows | Import | own |
| MOD | Models for IMOD | Export | own + matTomo |
| MRC | Medical Research Council format | Import/Export | own + matTomo |
| NRRD | Nearly Raw Raster Data | Import/Export | own + Projects:MATLABSlicerExampleModule and NRRD Format File Reader |
| STL | STereoLithography | Export | own + stlwrite |
| XML | Fiji Big Data Viewer | Import/Export | own |
| Zarr | OME Zarr v2/v3 | Import/Export | own, requires Python |
| MATLAB standard image and video formats | |||
| ASF | ASF Video File | Import | MATLAB |
| ASX | ASX Video File | Import | MATLAB |
| AVI | AVI Video File | Import/Export | MATLAB |
| BMP | Windows Bitmap | Import | MATLAB |
| CUR | Windows Cursor resources | Import | MATLAB |
| FTS, FITS | Flexible Image Transport System | Import | MATLAB |
| GIF | Graphics Interchange Format | Import | MATLAB |
| HDF | Hierarchical Data Format | Import | MATLAB |
| ICO | Windows Icon resources | Import | MATLAB |
| J2C, J2K | JPEG 2000 (raw codestream) | Import | MATLAB |
| JP2 | JPEG 2000 (Part 1) | Import | MATLAB |
| JPF, JPX | JPEG 2000 (Part 2) | Import | MATLAB |
| M4V | MPEG-4 Video | Import | MATLAB |
| MJ2 | Motion JPEG2000 Video | Import/Export | MATLAB |
| MOV | QuickTime movie | Import | MATLAB |
| MP4 | MPEG-4 Video | Import/Export | MATLAB |
| MPG | MPEG-1 Video | Import | MATLAB |
| PBM | Portable Bitmap | Import | MATLAB |
| PCX | Windows Paintbrush | Import | MATLAB |
| PGM | Portable Graymap | Import | MATLAB |
| PNG | Portable Network Graphics | Import/Export | MATLAB |
| PNM | Portable Any Map | Import | MATLAB |
| PPM | Portable Pixmap | Import | MATLAB |
| RAS | Sun Raster | Import | MATLAB |
| TIF, TIFF | Tagged Image File Format | Import/Export | MATLAB |
| WMV | Windows Media Video | Import | MATLAB |
| XWD | X Window Dump | Import | MATLAB |
|
Bio-Formats image reader triggered via the Bio checkbox within the Directory Contents panel of MIB | |||
| AL3D | Alicona 3D | Import | Bio-Formats |
| AM, AMIRAMESH, LABELS | Amira Mesh | Import | Bio-Formats |
| APL, MTB, TNB, OBSEP | Olympus CellR/APL | Import | Bio-Formats |
| ARF | Axon Raw Format | Import | Bio-Formats |
| C01 | Cellomics | Import | Bio-Formats |
| CZI | Zeiss CZI | Import | Bio-Formats |
| CXD | SimplePCI & HCImage | Import | Bio-Formats |
| DCM, DICOM | DICOM | Import | Bio-Formats |
| DM3 | Gatan Digital Micrograph | Import | Bio-Formats |
| DM4 | Gatan Digital Micrograph 2 | Import | Bio-Formats |
| DV, R3D | DeltaVision | Import | Bio-Formats |
| FITS | FITS (Flexible Image Transport System) | Import | Bio-Formats |
| FLEX | Evotec/PerkinElmer Opera Flex | Import | Bio-Formats |
| FLI | Lambert Instruments FLIM | Import | Bio-Formats |
| GEL | Amersham Biosciences Gel | Import | Bio-Formats |
| HDR | Analyze 7.5, Inveon , NIfTI , Unisoku | Import | Bio-Formats |
| ICS, IDS | ICS (Image Cytometry Standard) | Import | Bio-Formats |
| IMG | Burleigh, FEI, IMAGIC, NlfTI | Import | Bio-Formats |
| IMS | Bitplane Imaris | Import | Bio-Formats |
| IPL | IPLab | Import | Bio-Formats |
| IPM | IPLab-Mac | Import | Bio-Formats |
| IPW | ImagePro Workspace | Import | Bio-Formats |
| JP2 | JPEG 2000 | Import | Bio-Formats |
| L2D | Li-Cor L2D | Import | Bio-Formats |
| LEI | Leica LCS LEI | Import | Bio-Formats |
| LIF | Leica LAS AF LIF (Leica Image File Format) | Import | Bio-Formats |
| LIFF | Improvision Openlab LIFF | Import | Bio-Formats |
| LIM | LIM (Laboratory Imaging/Nikon) | Import | Bio-Formats |
| LSM | Zeiss LSM (Laser Scanning Microscope) 510/710 | Import | Bio-Formats |
| MDB | Zeiss LSM (Laser Scanning Microscope) 510/710 | Import | Bio-Formats |
| MNC | MINC MRI | Import | Bio-Formats |
| MNG | MNG (Multiple-image Network Graphics) | Import | Bio-Formats |
| MOV | QuickTime Movie | Import | Bio-Formats |
| MRC | MRC (Medical Research Council) | Import | Bio-Formats |
| MRW | Minolta MRW | Import | Bio-Formats |
| NAF | Hamamatsu Aquacosmos NAF | Import | Bio-Formats |
| ND | MetaMorph Stack (STK) | Import | Bio-Formats |
| ND2 | Nikon NIS-Elements ND2 | Import | Bio-Formats |
| NEF | NEF (Nikon Electronic Format) | Import | Bio-Formats |
| NRRD | NRRD (Nearly Raw Raster Data) | Import | Bio-Formats |
| OIB, OIF | Olympus FluoView FV1000 | Import | Bio-Formats |
| OME | OME-XML | Import | Bio-Formats |
| PGM | PGM (Portable Gray Map) | Import | Bio-Formats |
| PIC | Bio-Rad PIC | Import | Bio-Formats |
| PICT | PICT (Macintosh Picture) | Import | Bio-Formats |
| PSD | Adobe Photoshop PSD | Import | Bio-Formats |
| RAW | Improvision Openlab Raw, Bio-Rad PIC | Import | Bio-Formats |
| SDT | Becker & Hickl SPCImage | Import | Bio-Formats |
| SEQ | ImagePro Sequence | Import | Bio-Formats |
| STK | MetaMorph Stack (STK) | Import | Bio-Formats |
| SVS | Aperio SVS TIFF | Import | Bio-Formats |
| TIF | Tagged Image File Format | Import | Bio-Formats |
| VWS | TillPhotonics TillVision | Import | Bio-Formats |
| XDCE | InCell 1000 | Import | Bio-Formats |
| XML | Extensible Markup Language | Import | Bio-Formats |
| XV | Khoros VIFF (Visualization Image File Format) Bitmap | Import | Bio-Formats |
| XYS | VisiTech XYS | Import | Bio-Formats |
| ZVI | Zeiss AxioVision ZVI (Zeiss Vision Image) | Import | Bio-Formats |