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System Requirements
Contents:
Computer
MATLAB
Toolboxes
-------------------- Optional --------------------
Bio-Formats
BMxD filters
DipLib library
Edge Enhancing Coherence Filter
Fiji
Frangi Filter
Imaris
Membrane Click Tracker
Omero Server
Random Forest Classifier
Read NRRD
Segment-anything model
Supervoxels/Graphcut
Volume Rendering


Installation on Linux, contents

Requirements
  • MATLAB R2022a or newer (tested on R2022a, R2022b, R2023a)
  • Python 3.8, 3.9, 3.10; tested on 3.9
  • List of Python versions compatible with various MATLAB releases
  • Pytorch 1.10.0, (newer versions may not be compatible with Matlab installation)
  • CUDA compatible GPU (CUDA 11.1) is highly recommended, CPU can also be used but it is significantly slower

Python installation
Python for SAM is installed using miniconda, the instructions below are combined from recommendations from https://docs.anaconda.com/free/miniconda

  • Start terminal
  • Make a new directory for miniconda installation
    >> mkdir -p ~/miniconda3
  • Pick a suitable miniconda installation from https://repo.anaconda.com/miniconda
    Tested on Miniconda3-py39_23.11.0-2-Linux-x86_64.sh
    Type the following command by replacing the corresponding link with the desired one:
    >> wget https://repo.anaconda.com/miniconda/Miniconda3-py39_23.11.0-2-Linux-x86_64.sh -O ~/miniconda3/miniconda.sh
    >> bash ~/miniconda3/miniconda.sh -b -u -p ~/miniconda3
    >> rm -rf ~/miniconda3/miniconda.sh

  • Initialize for bash shell:
    >> ~/miniconda3/bin/conda init bash
  • Close and reopen terminal after this operation
  • Create a new environment for Python with SAM (replace python version with the installed)
    >> conda create --prefix ~/miniconda3/envs/sam4mib python=3.9
  • Activate the environment
    >> conda activate ~/miniconda3/envs/sam4mib


Install required python packages
requirements.txt
All the following steps can be substituted by installing required packages using requirements_linux.txt
Download requirements_linux.txt and use the following command to install packages:
(sam4mib)>> pip3 install -r requirements_linux.txt
After the installation, segment-anything can be located within "site-packages" directory of the sam4mib environment, e.g.
~/miniconda3/envs/sam4mib/lib/python3.9/site-packages
SAM requires python>=3.8, as well as pytorch>=1.7 and torchvision>=0.8;
SAM installation was tested with Pytorch 1.10.0 and CUDA version 11.1, the newer version may not be compatible.
The list of Pytorch installation option is available from https://pytorch.org/get-started/locally

  • Install pytorch:
    (sam4mib)>> pip3 install torch==1.10.0+cu111 torchvision==0.11.0+cu111 torchaudio==0.10.0 -f https://download.pytorch.org/whl/torch_stable.html
  • The following dependencies are necessary for mask post-processing, saving masks in COCO format, the example notebooks, and exporting the model in ONNX format
    (sam4mib)>> pip3 install opencv-python matplotlib onnxruntime onnx (sam4mib)>> pip3 install pycocotools
  • Install "onnxruntime-gpu" to make prediction on GPU
    (sam4mib)>> pip3 install onnxruntime-gpu
  • Install segment-anything model
    There are two options, either use a forked sam distribution or the recent distribition from facebookresearch, select one of these options:
    • Forked distibution tested with MIB:
      (sam4mib)>> pip3 install git+https://github.com/Ajaxels/segment-anything.git
    • Distribution available on facebookresearch:
      (sam4mib)>> pip3 install git+https://github.com/facebookresearch/segment-anything.git
    Destination!
    Segment-anything will be installed to "~/miniconda3/envs/sam4mib/lib/python3.9/site-packages"

    MIB configuration
    MIB configuration steps are similar to the SAM installation under Windows with the only difference in paths.
    • Define path of python3 installed in the specified environment (sam4mib):
      Menu->File->Preferences->External directories->Python installation path
      For example:
      - ~/miniconda3/envs/sam4mib/bin/python3
    • Define directory to store network architectures for DeepMIB;
      this location will be used to download checkpoints and onnx models.
    • Open SAM settings:
      Segmentation panel->Segment-anything model->Settings

    • Define location where segment-anything package was installed:
      "~/miniconda3/envs/sam4mib/lib/python3.9/site-packages"


    Testing sam4mib environment
    You can optionally test installation of SAM and sam4mib environment.
    • Check installation of pytorch in linux terminal:
      (sam4mib)>> python -c "import torch; print(torch.__version__)"
    • SAM distribution has amg.py under scripts subfolder, it can be used to test the installation under python.
      • Copy amg.py and notebook1.png (or any other png image) from SAM distribution into directory with the downloaded checkpoint (e.g. "sam_vit_b_01ec64.pth")
      • Start terminal in that directory and activate sam4mib environment
        >> conda activate ~/miniconda3/envs/sam4mib
      • Run the following command:
        (sam4mib)>> python amg.py --input notebook1.png --model-type 'vit_b' --output notebook2.png --checkpoint sam_vit_b_01ec64.pth
        to load the checkpoint file and do segmentation of the image, the results are placed to notebook2.png subfolder
    • Checksum test in terminal:
      >> md5sum sam_vit_b_01ec64.pth


    Remove sam4mib environment
    If you do not need mib4sam environment, you can follow the following steps to uninstall it from your system.
    • Start terminal
    • List the environments installed on your system by running the command:
      >> conda env list
    • Remove sam4mib environment:
      >> conda remove --name sam4mib --all
    • You can also remove miniconda completely:
      >> rm -rf ~/miniconda3


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