The same for MIB 3 under MATLAB and for the standalone versions:

• A splash screen is shown while MIB starts. The very first start of MIB and any of MIB tools takes a bit longer: MATLAB initializes the required libraries, the standalone version initializes the MATLAB Runtime
• MIB stores its settings in mib3.mat, see Configuration file. The settings and the user statistics of MIB 2 are not touched, so MIB 2 and MIB 3 can be used side by side
• Java is missing dialog: shown only when no Java is available to MIB (MATLAB and MATLAB Runtime R2026b and newer no longer include Java). MIB works without Java, but Bio-Formats, Fiji, Imaris and OMERO need it. Press Configure Java to let MIB find an installed Java or download one, or Instructions to open how to enable Java
  
  Missing Java dialog 1
  
  Missing Java dialog 2
• MIB user's statistics location dialog: asked once on each computer. MIB counts the tools you use; pick a folder that is available from every computer you use (for example, OneDrive or a network drive) and your statistics will add up across all of them. Statistics collected with MIB 2 are imported automatically. The location can be changed later from MIB->Home->Help->Your stats
  
  MIB user's statistics location dialog
• New version available dialog: a few seconds after the start MIB checks for updates and offers to download a newer version when there is one.
  Updates can also be checked later from MIB->Home->Help->Check for Update
  
  MIB3 Update dialog
• To use MIB with Fiji or OMERO, add their installation directories to MIB->Home->Preferences->External directories
  
• Access help from MIB->Home->Help or the MIB 3 user guide; see also tutorials

• Windows in C:\Users\Username\Matlab\mib3.mat
• MacOs in /Users/username/Matlab/mib3.mat
• Linux in /home/username/Matlab/mib3.mat

When the Matlab folder cannot be created, the system TEMP directory is used instead. The configuration file is automatically created after closing of MIB. The user statistics are kept in a separate file, see Configuration files in the MIB 3 user guide.
If MIB does not start check MATLAB path and/or delete the mib3.mat file.

MIB can be extended with various usable optional tools, unfortunately due to various reasons those tools can not be automatically installed and require manual installation or configuration.
Check the System Requirements section for optional software packages as segment-anything, BMxD filters, FIJI, and other options.

When using the BioFormats reader or FijiConnect you may get an error message that the amount of available Java heap space is too low:

This can be fixed by increasing Java Heap Space value in MATLAB

MIB under MATLAB environment

• Open MATLAB preferences
  
  Start MATLAB preferences dialog
• Navigate to
  MATLAB->General->Java Heap Memory
  Java Heap Memory settings
• Use the slider to increase Java Heap size to the desired value
• Press OK and restart MATLAB

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