Tools Menu
Back to MIB | User interface | Menu
Overview
Additional tools available in MIB.
Measure length
Allows measuring the length on the image.
Measure Tool
Based on the Image Measurement Utility
by Jan Neggers, Eindhoven University of Technology.
Enables various length measurements and generates corresponding intensity profiles.
See Measure Tool details.
Line measure
Measures the linear distance between two points.
Press and hold
Results will appear in a pop-up, printed to MATLAB’s main window, and copied to the clipboard
(paste with Ctrl+V).
Also accessible from the Toolbar.
Note
Pixel sizes are set in Dataset parameters.
Free hand measure
Similar to line measure, but allows arbitrary drawing of the measured distance.
Deep learning segmentation
Provides training of deep convolutional networks on user data and their use for image segmentation.
See Deep Learning details.
Classifiers
Two classifiers are available in MIB.
- Membrane detection: Uses a Random Forest classifier for automatic segmentation, based on Random Forest for Membrane Detection by Verena Kaynig and randomforest-matlab by Abhishek Jaiantilal. Works for membranes and other objects. See Random Forest help.
- Superpixels classification: Ideal for objects with distinct intensity properties. Calculates SLIC superpixels (2D) or supervoxels (3D) and classifies them using provided labels for objects and background. See Superpixels example.
Semi-automatic segmentation
Methods for automated image segmentation and object separation.
- Global thresholding
- Graphcut segmentation (recommended)
- Watershed segmentation
Object separation
Tools for separating objects in the current model, mask, or selection layers.
See more on object separation
Stereology
Generate a grid and counts model materials at grid line intersections.
Results can be exported to MATLAB or Excel.
See more on about Stereology
Wound healing assay
The wound healing assay is a microscopy-based technique used to study cell migration.
The Wound healing assay tool of MIB can be used to identify the wound area and measure its width.
See more about wound healing assay
Back to MIB | User interface | Menu